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pGEM-PAmCherry-RPA194
(Plasmid #247354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247354 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA194 (POLR1A)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3057
  • Entrez Gene
    POLR1A (a.k.a. A190, AFDCIN, HLD27, RPA1, RPA190, RPA194, RPO1-4, RPO14)
  • Tag / Fusion Protein
    • PAmCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-PAmCherry-RPA194 was a gift from Kazuhiro Maeshima (Addgene plasmid # 247354 ; http://n2t.net/addgene:247354 ; RRID:Addgene_247354)

  • For your References section:

    Transcriptional suppression of ribosomal DNA with phase separation. Ide S, Imai R, Ochi H, Maeshima K. Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 10.1126/sciadv.abb5953. Print 2020 Oct. 10.1126/sciadv.abb5953 PubMed 33055158
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