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pLEX_305_HA-ATXN1-DUX4
(Plasmid #247364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247364 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLEX_305
  • Backbone manufacturer
    Root Lab (Addgene #41390)
  • Backbone size w/o insert (bp) 7608
  • Total vector size (bp) 11136
  • Modifications to backbone
    Gateway cloned in the HA-ATXN1::DUX4 coding sequence
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    We have had a good experience using NEB 10beta.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-ATXN1::DUX4
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3528
  • Mutation
    Fusion between ATXN1 and DUX4
  • GenBank ID
    NM_001128164 NM_001306068
  • Promoter hPGK
  • Tag / Fusion Protein
    • 3x HA Tag (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer n/a insert too large - use whole plasmid sequencing
  • 3′ sequencing primer n/a insert too large - use whole plasmid sequencing
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For constitutive lentiviral expression by the hPGK promoter, with puromycin selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX_305_HA-ATXN1-DUX4 was a gift from Ross Okimoto (Addgene plasmid # 247364 ; http://n2t.net/addgene:247364 ; RRID:Addgene_247364)
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