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pQE30-His-spAb-T7 RNAP
(Plasmid #247369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247369 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3461
  • Total vector size (bp) 6324
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His-spAb-T7RNAP
  • Species
    Synthetic
  • Insert Size (bp)
    2904
  • Promoter T5-lacO
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    genScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.18.660355 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-His-spAb-T7 RNAP was a gift from Sebastian Maerkl (Addgene plasmid # 247369 ; http://n2t.net/addgene:247369 ; RRID:Addgene_247369)

  • For your References section:

    A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications. Lee P-W, Mottaghi SS, Lugnier MG, Maerkl SJ. bioRxiv 2025.06.18.660355 10.1101/2025.06.18.660355
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