Skip to main content
Addgene
Open Menu

pET-21(+)-His-zf438-NB23
(Plasmid #247372)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247372 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pET-21(+)
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6193
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His-zf438-NB23
  • Species
    Synthetic
  • Insert Size (bp)
    645
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.18.660355 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21(+)-His-zf438-NB23 was a gift from Sebastian Maerkl (Addgene plasmid # 247372 ; http://n2t.net/addgene:247372 ; RRID:Addgene_247372)

  • For your References section:

    A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications. Lee P-W, Mottaghi SS, Lugnier MG, Maerkl SJ. bioRxiv 2025.06.18.660355 10.1101/2025.06.18.660355
Related items: