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pCW-CKS2-HA
(Plasmid #247439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247439 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCW
  • Backbone manufacturer
    David Root, Addgene plasmid #41393
  • Backbone size w/o insert (bp) 7597
  • Total vector size (bp) 9278
  • Modifications to backbone
    Substitution of everything between tight TRE promoter and hPGK promoter with our inserts
  • Vector type
    Mammalian Expression, Lentiviral ; Dox-inducible
  • Selectable markers
    Puromycin ; EGFP expression upon doxycycline treatment

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CKS2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    237
  • Entrez Gene
    Cks2 (a.k.a. 1110038L14Rik, CKSHS2)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    IRES2:EGFP
  • Insert Size (bp)
    1296

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.06.657372 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW-CKS2-HA was a gift from Richard Treisman (Addgene plasmid # 247439 ; http://n2t.net/addgene:247439 ; RRID:Addgene_247439)

  • For your References section:

    MRTF-dependent cytoskeletal dynamics drive efficient cell cycle progression. Nielsen JC, Benito-Jardon M, Petrela NC, Diring J, Bellamy S, Treisman R. J Cell Sci. 2025 Nov 7:jcs.264444. doi: 10.1242/jcs.264444. 10.1242/jcs.264444 PubMed 41200793
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