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pEF1-EGFP-coilin-FRT
(Plasmid #247448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247448 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEF5
  • Backbone manufacturer
    Invitrogen, Cat#V602020
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    coilin (COIL)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2489
  • Entrez Gene
    COIL (a.k.a. CLN80, p80-coilin)
  • Promoter EF1α
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1-EGFP-coilin-FRT was a gift from Kazuhiro Maeshima (Addgene plasmid # 247448 ; http://n2t.net/addgene:247448 ; RRID:Addgene_247448)

  • For your References section:

    1,6-hexanediol rapidly immobilizes and condenses chromatin in living human cells. Itoh Y, Iida S, Tamura S, Nagashima R, Shiraki K, Goto T, Hibino K, Ide S, Maeshima K. Life Sci Alliance. 2021 Feb 3;4(4):e202001005. doi: 10.26508/lsa.202001005. Print 2021 Apr. 10.26508/lsa.202001005 PubMed 33536240
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