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PB-EF1-H4Δ1-19-HaloTag-IRES-Neo
(Plasmid #247451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247451 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPB-PGKNeo
  • Backbone manufacturer
    Junji Takeda lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H4 (H4C1)
  • Alt name
    H4 (H4C1), Δ1-19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1206
  • Mutation
    H4Δ1-19 lacks the initial 19 residues at the N-terminus.
  • Entrez Gene
    H4C1 (a.k.a. H4-16, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A)
  • Promoter EF1α
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-EF1-H4Δ1-19-HaloTag-IRES-Neo was a gift from Kazuhiro Maeshima (Addgene plasmid # 247451 ; http://n2t.net/addgene:247451 ; RRID:Addgene_247451)

  • For your References section:

    Behaviors of nucleosomes with mutant histone H4s in euchromatic domains of living human cells. Semeigazin A, Iida S, Minami K, Tamura S, Ide S, Higashi K, Toyoda A, Kurokawa K, Maeshima K. Histochem Cell Biol. 2024 Jul;162(1-2):23-40. doi: 10.1007/s00418-024-02293-x. Epub 2024 May 14. 10.1007/s00418-024-02293-x PubMed 38743310
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