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pAAVS-NDi-H1.2_WT-Halo
(Plasmid #247484)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247484 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAVS1-Ndi-CRISPRi
  • Backbone manufacturer
    Addgene, #73498
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H1.2 (HIST1H1C)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1598
  • Entrez Gene
    H1-2 (a.k.a. H1.2, H1C, H1F2, H1s-1, HIST1H1C)
  • Promoter TRE3G
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.05.641622 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS-NDi-H1.2_WT-Halo was a gift from Kazuhiro Maeshima (Addgene plasmid # 247484 ; http://n2t.net/addgene:247484 ; RRID:Addgene_247484)

  • For your References section:

    Linker histone H1 functions as a liquid-like glue to organize chromatin in living human cells. Shimazoe MA, Huertas J, Phillips C, Ide S, Tamura S, Farr S, Ashwin SS, Sasai M, Collepardo-Guevara R, Maeshima K. Sci Adv. 2026 Apr 10;12(15):eaec9801. doi: 10.1126/sciadv.aec9801. Epub 2026 Apr 8. 10.1126/sciadv.aec9801 PubMed 41950317
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