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pGEM_H1.2_mAID_mClover
(Plasmid #247486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247486 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    mAID-mClover and pGEM-T Easy
  • Backbone manufacturer
    Addgene, #124496 and Promega A1360
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H1.2 (HIST1H1C)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    H1-2 (a.k.a. H1.2, H1C, H1F2, H1s-1, HIST1H1C)
  • Tags / Fusion Proteins
    • mAID
    • mClover

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.05.641622 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM_H1.2_mAID_mClover was a gift from Kazuhiro Maeshima (Addgene plasmid # 247486 ; http://n2t.net/addgene:247486 ; RRID:Addgene_247486)

  • For your References section:

    Linker histone H1 functions as a liquid-like glue to organize chromatin in living human cells. Shimazoe MA, Huertas J, Phillips C, Ide S, Tamura S, Farr S, Ashwin SS, Sasai M, Collepardo-Guevara R, Maeshima K. Sci Adv. 2026 Apr 10;12(15):eaec9801. doi: 10.1126/sciadv.aec9801. Epub 2026 Apr 8. 10.1126/sciadv.aec9801 PubMed 41950317
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