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pHR-Gal4UAS-GFP-PGK-BFP
(Plasmid #247506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247506 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL4/UAS expression of GFP and constitutive expression of BFP
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Wendell Lim (pHR backbone Gal4UAS and mCMV promoter, Addgene #79119; PGK promoter, Addgene #79120).

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an indel in the PGK promoter that differs between the Addgene NGS sequence and the depositor reference; the depositor confirms that this is not of concern for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-Gal4UAS-GFP-PGK-BFP was a gift from Wendell Lim (Addgene plasmid # 247506 ; http://n2t.net/addgene:247506 ; RRID:Addgene_247506)

  • For your References section:

    Programming tissue-sensing T cells that deliver therapies to the brain. Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA. Science. 2024 Dec 6;386(6726):eadl4237. doi: 10.1126/science.adl4237. Epub 2024 Dec 6. 10.1126/science.adl4237 PubMed 39636984
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