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pHR_PGK_ anti-NRCAM 12950 (VL + VH) synNotch
(Plasmid #247509)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247509 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synNotch binding to NrCAM (neural cell adhesion molecule)
  • Species
    H. sapiens (human), Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wendell Lim (pHR backbone and PGK promoter, Addgene #79120); University of Toronto (anti-NRCAM 12950).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a discrepancy between the Addgene NGS sequence and the depositor's reference: SNP in synNotch E654D. The depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR_PGK_ anti-NRCAM 12950 (VL + VH) synNotch was a gift from Wendell Lim (Addgene plasmid # 247509 ; http://n2t.net/addgene:247509 ; RRID:Addgene_247509)

  • For your References section:

    Programming tissue-sensing T cells that deliver therapies to the brain. Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA. Science. 2024 Dec 6;386(6726):eadl4237. doi: 10.1126/science.adl4237. Epub 2024 Dec 6. 10.1126/science.adl4237 PubMed 39636984
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