Skip to main content
Addgene
Open Menu

cTurboID-myc_pHygGate100
(Plasmid #247583)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247583 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pEarleyGate
  • Modifications to backbone
    In this construct, the phosphinothricin acetyltransferase gene (BASTA) was disrupted by insertion of an aminoglycoside phosphotransferase gene, thereby conferring hygromycin resistance. This modification was performed by Dr. Yu Tang in Dr. Yangnan Gu’s lab.
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cTurboID
  • Insert Size (bp)
    726
  • Tag / Fusion Protein
    • MYC

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds a transposable element (WP_010723085.1) inserted in the plasmid backbone upstream of the KanR feature.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cTurboID-myc_pHygGate100 was a gift from Juan Dong (Addgene plasmid # 247583 ; http://n2t.net/addgene:247583 ; RRID:Addgene_247583)

  • For your References section:

    Split-YFP-coupled interaction-dependent TurboID identifies new functions of basal cell polarity in Arabidopsis. Huang A, Zhang J, Liu Z, Schoen V, Verma D, Zheng H, Pedmale UV, Dong J. Proc Natl Acad Sci U S A. 2025 Aug 12;122(32):e2502445122. doi: 10.1073/pnas.2502445122. Epub 2025 Aug 6. 10.1073/pnas.2502445122 PubMed 40768356
Related items: