pNH604-Padh-OsTIR1(F74G)
(Plasmid
#247606)
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PurposeExpression of OsTIR1
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepNH604
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1
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SpeciesOryza sativa
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MutationF74G
- Promoter ADH1
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also known as pSS1361. Please visit https://doi.org/10.1101/2025.09.24.678194 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH604-Padh-OsTIR1(F74G) was a gift from Sebastian Schuck (Addgene plasmid # 247606 ; http://n2t.net/addgene:247606 ; RRID:Addgene_247606) -
For your References section:
An optimised system for rapid auxin-inducible protein degradation in budding yeast. Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, Friemel N, Schuck S. bioRxiv 2025.09.24.678194 10.1101/2025.09.24.678194