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TEVEp_pRK793
(Plasmid #247672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247672 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRK793
  • Backbone manufacturer
    David Waugh, Addgene plasmid #8827
  • Backbone size w/o insert (bp) 5464
  • Total vector size (bp) 7381
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The BL21(DE3)-RIL strain should be used for expression. Strain contains chloramphenicol resistance.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MBP-ENLYFES-7xHIS-TEVEp
  • Alt name
    TEV-PE10
  • Alt name
    tobacco etch virus protease
  • Insert Size (bp)
    1917
  • Mutation
    S120R, D148R, T173A, N177K, M218I, S219P
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • MBP (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The TEVEp (TEV-PE10) insert was optimized for e. coli expression and based on: L. Yi, M.C. Gebhard, Q. Li, J.M. Taft, G. Georgiou, & B.L. Iverson, Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries, Proc. Natl. Acad. Sci. U.S.A. 110 (18) 7229-7234, https://doi.org/10.1073/pnas.1215994110 (2013).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TEVEp_pRK793 was a gift from Carl Denard (Addgene plasmid # 247672 ; http://n2t.net/addgene:247672 ; RRID:Addgene_247672)

  • For your References section:

    PERRC: Protease Engineering with Reactant Residence Time Control. Nelson S, Gaza J, Ajayebi S, Masse R, Pho R, Scutero C, Martinusen S, Long L, Menezes A, Perez A, Denard C. ACS Synth Biol. 2025 Jun 20;14(6):2241-2253. doi: 10.1021/acssynbio.5c00154. Epub 2025 May 19. 10.1021/acssynbio.5c00154 PubMed 40388903
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