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pcDNA3.1-HA-dnGFP-OGT(13, K852A)
(Plasmid #247678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247678 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5375
  • Total vector size (bp) 8975
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-dnGFP-OGT(13, K852A)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3598
  • Mutation
    K852A mutation made in OGT
  • Entrez Gene
    OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
  • Promoter T7
  • Tag / Fusion Protein
    • HA-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sgfl (not destroyed)
  • 3′ cloning site Sgsl (not destroyed)
  • 5′ sequencing primer T7 fwd
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HA-dnGFP-OGT(13, K852A) was a gift from Christina Woo (Addgene plasmid # 247678 ; http://n2t.net/addgene:247678 ; RRID:Addgene_247678)

  • For your References section:

    Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc. Mody AC, Ramirez DH, Woo CM. Cell Chem Biol. 2025 Dec 18;32(12):1486-1502.e7. doi: 10.1016/j.chembiol.2025.11.003. Epub 2025 Dec 8. 10.1016/j.chembiol.2025.11.003 PubMed 41365299
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