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pAAV-EF1a>WAChR_1m-G-Soma-E-HA-P2A-mScarlet; WPRE3-BGH polyA; KanR
(Plasmid #247728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247728 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV[Exp]
  • Backbone manufacturer
    Vectorbuilder
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WAChR_1m-G-Soma-E-HA-P2A-mScarlet
  • Species
    Synthetic
  • Mutation
    S122L/F240A
  • Promoter EF1a
  • Tag / Fusion Protein
    • mScarlet (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.12.675947 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a>WAChR_1m-G-Soma-E-HA-P2A-mScarlet; WPRE3-BGH polyA; KanR was a gift from Alexander Naka (Addgene plasmid # 247728 ; http://n2t.net/addgene:247728 ; RRID:Addgene_247728)

  • For your References section:

    WAChRs are excitatory opsins sensitive to indoor lighting. Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A. bioRxiv 2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947 10.1101/2025.09.12.675947
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