pcDNA3.1_ECFP
(Plasmid
#247760)
-
Purposemammalian expression of ECFP
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepcDNA3.1
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6093
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameECFP
-
Insert Size (bp)720
-
GenBank ID
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1_ECFP was a gift from Alan Ashworth (Addgene plasmid # 247760 ; http://n2t.net/addgene:247760 ; RRID:Addgene_247760) -
For your References section:
An engineered cysteine sensor optimized for high-throughput screening identifies regulators of intracellular thiol levels. Abrams RPM, Donahue RG, Ma J, Mao Y, Diolaiti ME, Ashworth A. Cell Chem Biol. 2025 Nov 10:S2451-9456(25)00343-5. doi: 10.1016/j.chembiol.2025.10.006. 10.1016/j.chembiol.2025.10.006 PubMed 41218609