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anti-FLAG-CAR (human, optimised, tonic-signal-free)
(Plasmid #247778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247778 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDH-mPLUM
  • Backbone size w/o insert (bp) 7729
  • Total vector size (bp) 9328
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    mPLUM

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    antiFLAG-CAR4
  • Species
    Synthetic
  • Insert Size (bp)
    1507
  • Promoter EF-1α
  • Tag / Fusion Protein
    • Myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TAAGTTTAAAGCTCAGGTCGAGACCGG
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anti-FLAG-CAR (human, optimised, tonic-signal-free) was a gift from Clare Slaney (Addgene plasmid # 247778 ; http://n2t.net/addgene:247778 ; RRID:Addgene_247778)

  • For your References section:

    Optimised modular anti-FLAG CAR T cells for solid tumor therapy. Zhang X, Xu R, Zorin D, Pappas EG, Tang J, Bai Y, Qin VM, von Scheidt B, Huang R, Kulakowska W, Darido C, Darcy PK, Kershaw MH, Slaney CY. Clin Transl Immunology. 2025 Aug 21;14(8):e70046. doi: 10.1002/cti2.70046. eCollection 2025. 10.1002/cti2.70046 PubMed 40851786
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