pHLEGM3
(Plasmid #24782)

• Depositing Lab: Tanya Parish
• Publication: Carroll et al Appl Environ Microbiol. 2005 Jun . 71(6):3077-84.

Backbone

• Vector backbone: pFLAME4
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ptet-Gfp-ASV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: 1. Gfp-LAA obtained from Marian Blokpoel 2. TetR obtained from Ambrose Cheung 3. Ag85a promoter obtained from Edith Machowski

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed]

2.

2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]