pLEX307-SFB-ZNF451
(Plasmid
#247909)
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PurposeMammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged ZNF451
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLEX307-SFB-N
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZNF451
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SpeciesH. sapiens (human)
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Entrez GeneZNF451 (a.k.a. COASTER, dJ417I1.1)
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Tag
/ Fusion Protein
- S protein-Flag-Streptavidin binding peptide (SFB) (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-SFB-ZNF451 was a gift from Junjie Chen & Huimin Zhang (Addgene plasmid # 247909 ; http://n2t.net/addgene:247909 ; RRID:Addgene_247909) -
For your References section:
RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons. Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, Pommier Y, Chen J. Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. Epub 2023 Dec 6. 10.1126/sciadv.adi6681 PubMed 38055811