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pLEX307-RAD54L2-D1-86-SFB
(Plasmid #247913)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247913 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLEX307-SFB-C
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAD54L2
  • Species
    H. sapiens (human)
  • Mutation
    deletion of amino acid 1-86
  • Entrez Gene
    RAD54L2 (a.k.a. ARIP4, HSPC325, SRISNF2L)
  • Tag / Fusion Protein
    • S protein-Flag-Streptavidin binding peptide (SFB) (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-RAD54L2-D1-86-SFB was a gift from Junjie Chen & Huimin Zhang (Addgene plasmid # 247913 ; http://n2t.net/addgene:247913 ; RRID:Addgene_247913)

  • For your References section:

    RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons. Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, Pommier Y, Chen J. Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. Epub 2023 Dec 6. 10.1126/sciadv.adi6681 PubMed 38055811
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