pFastBac1-His-GFP-RAD54L2
(Plasmid
#247924)
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PurposeInsect expression construct for N-terminal His-GFP-tagged RAD54L2
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepFastBac1
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAD54L2
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SpeciesH. sapiens (human)
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Entrez GeneRAD54L2 (a.k.a. ARIP4, HSPC325, SRISNF2L)
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Tag
/ Fusion Protein
- His-GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac1-His-GFP-RAD54L2 was a gift from Junjie Chen & Huimin Zhang (Addgene plasmid # 247924 ; http://n2t.net/addgene:247924 ; RRID:Addgene_247924) -
For your References section:
RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons. Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, Pommier Y, Chen J. Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. Epub 2023 Dec 6. 10.1126/sciadv.adi6681 PubMed 38055811