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pMH180_pDEST_CMV_UBR5_Flag
(Plasmid #247931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247931 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDEST-FLAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBR5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8400
  • Entrez Gene
    UBR5 (a.k.a. DD5, EDD, EDD1, HYD, NEDSBH)
  • Tag / Fusion Protein
    • FLAG (DYKDDDDK) (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. The Addgene NGS result finds alternative amino acids in the insert ORF downstream of FLAG and K503R in hUBR5 (NP_056986.2).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH180_pDEST_CMV_UBR5_Flag was a gift from Benjamin Ebert & Jonathan Tsai (Addgene plasmid # 247931 ; http://n2t.net/addgene:247931 ; RRID:Addgene_247931)

  • For your References section:

    UBR5 forms ligand-dependent complexes on chromatin to regulate nuclear hormone receptor stability. Tsai JM, Aguirre JD, Li YD, Brown J, Focht V, Kater L, Kempf G, Sandoval B, Schmitt S, Rutter JC, Galli P, Sandate CR, Cutler JA, Zou C, Donovan KA, Lumpkin RJ, Cavadini S, Park PMC, Sievers Q, Hatton C, Ener E, Regalado BD, Sperling MT, Slabicki M, Kim J, Zon R, Zhang Z, Miller PG, Belizaire R, Sperling AS, Fischer ES, Irizarry R, Armstrong SA, Thoma NH, Ebert BL. Mol Cell. 2023 Aug 3;83(15):2753-2767.e10. doi: 10.1016/j.molcel.2023.06.028. Epub 2023 Jul 20. 10.1016/j.molcel.2023.06.028 PubMed 37478846
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