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pET20b-His6-LONP1-Pore Loop 2 mutant (Y599A)
(Plasmid #247939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247939 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET20b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LONP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2540
  • Mutation
    encodes aa 115-959, with Y599A
  • Entrez Gene
    LONP1 (a.k.a. CODASS, LON, LONP, LonHS, PIM1, PRSS15, hLON)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b-His6-LONP1-Pore Loop 2 mutant (Y599A) was a gift from Gabriel Lander (Addgene plasmid # 247939 ; http://n2t.net/addgene:247939 ; RRID:Addgene_247939)

  • For your References section:

    Structures of the human LONP1 protease reveal regulatory steps involved in protease activation. Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC. Nat Commun. 2021 May 28;12(1):3239. doi: 10.1038/s41467-021-23495-0. 10.1038/s41467-021-23495-0 PubMed 34050165
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