pDIV1049
(Plasmid
#247970)
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PurposeCRISPR-Cas9 vector containing a 20 nt protospacer sequence to target mCitrine. pyrG marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepFC330
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Vector typeCRISPR
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9-AMA1-mCit-gRNA-pyrG-Ori-AmpR
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gRNA/shRNA sequenceUnknown
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SpeciesOther
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Mutationnone
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.13.612835 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDIV1049 was a gift from Uffe Mortensen (Addgene plasmid # 247970 ; http://n2t.net/addgene:247970 ; RRID:Addgene_247970)