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pART7
(Plasmid #247985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247985 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pART7
  • Backbone manufacturer
    Andrew Gleave
  • Backbone size (bp) 5042
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pART7 is a synthetic cloning vector harbouring a CaMV 35S promoter and a multi cloning site for inserting genes intended to be transiently expressed in plants. Two NotI restriction sites allow to excise the cloned gene for subcloning into a binary vector. pART7 is a derivative of pGEM-9zf- (GenBank X65312). See the Supplemental Document under Resource Information for more details, including annotated maps.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pART7 was a gift from Andrew Gleave & Christoph Plieth (Addgene plasmid # 247985 ; http://n2t.net/addgene:247985 ; RRID:Addgene_247985)

  • For your References section:

    A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Gleave AP. Plant Mol Biol. 1992 Dec;20(6):1203-7. PubMed 1463857
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