pART27
(Plasmid #247986)

• Purpose: (Empty Backbone) Binary vector to receive a NotI fragment from pART7 with a gene to be stably expressed in plants via agrobacterium-mediated transformation.
• Depositing Labs: Andrew Gleave, Christoph Plieth
• Publication: Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Backbone

• Vector backbone: pART27
• Backbone manufacturer: Andrew Gleave
• Backbone size (bp): 11612
• Vector type: Plant Expression
• Selectable markers: lacZ (blue-white screening) and Kanamycin (in plants)

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • Description of plasmids

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pART27 is the binary vector corresponding to pART7. It harbours a transfer DNA gene cassette (tDNA) between the left and right borders to be integrated into the genome of higher plants by Agrobacterium-mediated gene transfer. A single NotI restriction site allows to integrate a NotI-restricted gene cassette from pART7 into the tDNA of pART27. pART27 is a derivative of pMON530 (Rogers et al., 1987; doi: 10.1016/0076-6879(87)53058-0). See the Supplemental Document under Resource Information for more details, including annotated maps.