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V5-BS2-TRFLE-NLS
(Plasmid #247991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247991 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    d0
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BS2-TRFLE Esterase
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    1566
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 Tag (N terminal on insert)
    • NLS (Nuclear Localization Sequence) (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.26434/chemrxiv-2025-d6bzr for ChemRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V5-BS2-TRFLE-NLS was a gift from Jeffrey Martell (Addgene plasmid # 247991 ; http://n2t.net/addgene:247991 ; RRID:Addgene_247991)

  • For your References section:

    Directed Evolution of Enzymes for Bioorthogonal Chemistry Using Acid Chloride Proximity Labeling. Ogorek AN, Pani S, Mertick-Sykes EJ, Momirov J, Lao Y, Mejia FB, Chan RST, Huang X, Dickinson BC, Martell JD. ACS Cent. Sci. 2026, 10.1021/acscentsci.5c01746
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