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pET28-hisSumo-KE-Des27.7
(Plasmid #248003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248003 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KE-Des27.7
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • 12xHis-bdSumo (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-hisSumo-KE-Des27.7 was a gift from Sarel Fleishman (Addgene plasmid # 248003 ; http://n2t.net/addgene:248003 ; RRID:Addgene_248003)

  • For your References section:

    Complete computational design of high-efficiency Kemp elimination enzymes. Listov D, Vos E, Hoffka G, Hoch SY, Berg A, Hamer-Rogotner S, Dym O, Kamerlin SCL, Fleishman SJ. Nature. 2025 Jul;643(8074):1421-1427. doi: 10.1038/s41586-025-09136-2. Epub 2025 Jun 18. 10.1038/s41586-025-09136-2 PubMed 40533551
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