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Teadlight-T2-8xGTIIc-IVS-Syn21-AchillesNlsPest-p10
(Plasmid #248043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248043 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTol2
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 4417
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8xGTIIC TEAD-binding synthetic promoter driving the expression of Achilles-NLS-PEST
  • Alt name
    TEADlight
  • Insert Size (bp)
    1744

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer Seq-Tol2 - GTCGACcagaggtgtaaagtac
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from : https://www.addgene.org/34615/ and https://www.addgene.org/153528/

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Teadlight-T2-8xGTIIc-IVS-Syn21-AchillesNlsPest-p10 was a gift from Christophe Marcelle (Addgene plasmid # 248043 ; http://n2t.net/addgene:248043 ; RRID:Addgene_248043)

  • For your References section:

    TEADlight, a bright dynamic reporter for live detection of YAP/TAZ-TEAD signaling. Morin V, Le Toquin Y, Tepordei D, Marcelle C, Delaune E. Dev Biol. 2025 Sep;525:194-205. doi: 10.1016/j.ydbio.2025.06.007. Epub 2025 Jun 6. 10.1016/j.ydbio.2025.06.007 PubMed 40482695
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