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pLVX-TetOne-puro-atpG
(Plasmid #248067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248067 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-TetOne-puro
  • Backbone manufacturer
    TakaraBio
  • Backbone size w/o insert (bp) 9138
  • Total vector size (bp) 10125
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    atpG
  • Species
    E. coli
  • Insert Size (bp)
    918
  • Mutation
    codon-optimized for expression in human cells
  • Entrez Gene
    atpG (a.k.a. b3733, ECK3726, papC, uncG)
  • Promoter TRE3GS
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.12.670003 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOne-puro-atpG was a gift from Denis Titov (Addgene plasmid # 248067 ; http://n2t.net/addgene:248067 ; RRID:Addgene_248067)

  • For your References section:

    Genetically encoded tool for manipulation of ATP/ADP ratio in human cells. Ekvik AE, Kober MM, Titov DV. bioRxiv [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 10.1101/2025.08.12.670003. 10.1101/2025.08.12.670003 PubMed 40894546
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