PCS2-CIB1-RFP-IFT88
(Plasmid #248077)

• Purpose: For use in light-induced IFT88 protein clustering through the interaction with CRY2
• Depositing Lab: Heidi Hehnly
• Publication: Hehnly lab plasmids (unpublished)

Backbone

• Vector backbone: PCS2
• Backbone manufacturer: Dave Turner lab (UMich)
• Backbone size w/o insert (bp): 4090
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CIB1
• Alt name: cryptochrome-interacting basic-helix-loop-helix 1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 517
• Mutation: none
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • RFP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: sp6

Gene/Insert 2

• Gene/Insert name: IFT88
• Alt name: intraflagellar transport 88
• Species: M. musculus (mouse)
• Insert Size (bp): 2478
• Mutation: none
• Entrez Gene: Ift88 (a.k.a. Tg737, Tg737Rpw, TgN737Rpw, Ttc10, flexo, fxo, orpk, polaris)
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • RFP (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • PCS2-CIB1-RFP-IFT88.gb
• A portion of this plasmid was derived from a plasmid made by: A portion of this plasmid was derived from a plasmid made by Addgene plasmid 140573. Sub cloned IFT88 from a murine cDNA library.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry