Sox17::CRY2-GFP
(Plasmid
#248078)
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PurposeExpression of Sox17-driven CRY2–GFP fusion protein for optogenetic control
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepTol2
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Backbone manufacturerKoichi Kawakami Lab, National Institute of Genetics (Japan)
- Backbone size w/o insert (bp) 9910
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCRY2
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Alt nameCryptochrome 2
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1512
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Mutationnone
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter Sox17
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Rev
- 3′ sequencing primer M13 Fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byA portion of this plasmid was derived from a plasmid made by Addgene plasmid 58368.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sox17::CRY2-GFP was a gift from Heidi Hehnly (Addgene plasmid # 248078 ; http://n2t.net/addgene:248078 ; RRID:Addgene_248078)