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LNPL1093-piggybac-Dox-inducible-CasRx-QK634-UnirapR-IRES-mCherry
(Plasmid #248111)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248111 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    piggybac
  • Backbone size w/o insert (bp) 5646
  • Total vector size (bp) 9234
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasRx-UnirapR
  • Alt name
    RfxCas13d-UnirapR
  • Species
    Synthetic
  • Insert Size (bp)
    3588
  • Promoter Tet inducible

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The vector does not contain rTTA; therefore, the cell line needs to constitutively express rTTA for this system to work.

Please visit https://doi.org/10.1101/2025.04.21.649818 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LNPL1093-piggybac-Dox-inducible-CasRx-QK634-UnirapR-IRES-mCherry was a gift from Jared Toettcher (Addgene plasmid # 248111 ; http://n2t.net/addgene:248111 ; RRID:Addgene_248111)

  • For your References section:

    Multimodal control of Cas13 activity through domain insertion at an allosteric hotspot. Zhu L, Nguyen LT, Bell AG, Gillmann KM, Oatman H, Hariri J, Myhrvold C, Toettcher JE. bioRxiv 2025.04.21.649818 10.1101/2025.04.21.649818
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