pNeonGreen-C1-Nir1-LNS2-K820E
(Plasmid #248112)

• Purpose: PILS-Nir1 K820E non-PA binding negative control
• Depositing Lab: Gerry Hammond
• Publication: Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9.

Backbone

• Vector backbone: pNeonGreen-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PITPNM3
• Alt name: Nir1
• Species: H. sapiens (human)
• Insert Size (bp): 792
• Mutation: amino acids 631-894. Contains K820E PA binding mutation and natural variant V855A.
• Entrez Gene: PITPNM3 (a.k.a. ACKR6, CORD5, NIR1, RDGBA3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • NeonGreen-GGSGG (N terminal on insert)
  • neonGreen (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SV40pArev

Resource Information

• Supplemental Documents:
  • pNeonGreen-C1-Nir1-LNS2-K820E.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Nir1 sequence corresponds to a rare human allele (17-6455609-G-A (GRCh38) in the gnomAD database) with an A885V missense mutation relative to the reference genome. There is no known phenotype associated with mutation of this residue, and it is not predicted to disrupt membrane interactions. The residue is also not conserved between Nir orthologues.