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P23_vanR_Ptec_mCherry
(Plasmid #248114)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248114 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLp_3050sNuc
  • Backbone manufacturer
    Addgene 122030
  • Backbone size w/o insert (bp) 2685
  • Total vector size (bp) 4590
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry red fluorescent protein
  • Species
    Synthetic; Discosoma spp
  • Insert Size (bp)
    711
  • Promoter Ptec flanked by vanO operators

Cloning Information for Gene/Insert 1

  • Cloning method Gene Synthesis
  • 5′ sequencing primer aagttgagcaagagatcg
  • 3′ sequencing primer aaactcacgttaagggat
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    VanR repressor
  • Species
    Synthetic; Bacterial origin
  • Insert Size (bp)
    735
  • Promoter P23

Cloning Information for Gene/Insert 2

  • Cloning method Gene Synthesis
  • 5′ sequencing primer tagacgcatagatctcgct
  • 3′ sequencing primer ttcgatctctacgaacga
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P23_vanR_Ptec_mCherry was a gift from Shrikrishnan Sankaran (Addgene plasmid # 248114 ; http://n2t.net/addgene:248114 ; RRID:Addgene_248114)

  • For your References section:

    Encapsulation-enhanced switchable protein release from engineered probiotic lactobacilli. Blanch-Asensio M, Tadimarri VS, Martinez R, Dahiya GS, Duong CN, Lale R, Sankaran S. J Control Release. 2025 Sep 24;387:114264. doi: 10.1016/j.jconrel.2025.114264. 10.1016/j.jconrel.2025.114264 PubMed 41005743
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