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pLVX-EF1_-FCRL3-IRES-puro
(Plasmid #248148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248148 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-EF1_-IRES-Puro
  • Backbone size w/o insert (bp) 8899
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FCRL3
  • Alt name
    CD307
  • Alt name
    SPAP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2207
  • Entrez Gene
    FCRL3 (a.k.a. CD307c, FCRH3, IFGP3, IRTA3, MAIA, SPAP2)
  • Promoter EF1_

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Variant of FCRL3 sequence; rs7522061 SNP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1_-FCRL3-IRES-puro was a gift from Silvia Monticelli (Addgene plasmid # 248148 ; http://n2t.net/addgene:248148 ; RRID:Addgene_248148)

  • For your References section:

    FCRL3 is an immunoregulatory receptor that restrains the activation of human memory T lymphocytes. Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G, Monticelli S. J Exp Med (2026) 223 (1): e20242474. 10.1084/jem.20242474
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