pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro
(Plasmid
#248149)
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PurposeLentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-687; first 93 residues), fused to the extracellular domain of EGFR.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLVX-EF1_-IRES-Puro
- Backbone size w/o insert (bp) 8899
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFR-FCRL3
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SpeciesH. sapiens (human)
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Insert Size (bp)2280
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Entrez GeneFCRL3 (a.k.a. CD307c, FCRH3, IFGP3, IRTA3, MAIA, SPAP2)
- Promoter EF1_
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro was a gift from Silvia Monticelli (Addgene plasmid # 248149 ; http://n2t.net/addgene:248149 ; RRID:Addgene_248149) -
For your References section:
FCRL3 is an immunoregulatory receptor that restrains the activation of human memory T lymphocytes. Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G, Monticelli S. J Exp Med (2026) 223 (1): e20242474. 10.1084/jem.20242474