pLV-Hygro-EF1A-HA-PARP1-3GS-TurboC
(Plasmid
#248158)
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PurposePARP1 with split TurboID at C-term
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneLentivirus
- Total vector size (bp) 12737
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePARP1
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SpeciesH. sapiens (human)
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Insert Size (bp)3039
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GenBank IDNM_001618 NP_001609
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Entrez GenePARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- Split TurboID: C (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-Hygro-EF1A-HA-PARP1-3GS-TurboC was a gift from Robert Sobol (Addgene plasmid # 248158 ; http://n2t.net/addgene:248158 ; RRID:Addgene_248158) -
For your References section:
Replication-associated base excision repair/single-strand break repair regulates PARG inhibitor response via the PRMT1/PRMT5/ATR axis. Ibrahim M, Roos WP, Schwartz JC, Khan MM, Al-Rahahleh RQ, Beers LA, Pearson CR, Langham KT, Boyang L, Clark J, Hayat F, Fang Q, Koczor CA, Migaud ME, Sobol RW. NAR Cancer. 2025 Dec 31;7(4):zcaf057. doi: 10.1093/narcan/zcaf057. eCollection 2025 Dec. 10.1093/narcan/zcaf057 PubMed 41480640