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pB-Orthogonal_LTR5Hs_CARGO
(Plasmid #248159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248159 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    piggyBac
  • Total vector size (bp) 13812
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Array of guide RNAs
  • gRNA/shRNA sequence
    LTR5Hs elements
  • Species
    Synthetic
  • Tag / Fusion Protein
    • mCherry

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-Orthogonal_LTR5Hs_CARGO was a gift from Joanna Wysocka (Addgene plasmid # 248159 ; http://n2t.net/addgene:248159 ; RRID:Addgene_248159)

  • For your References section:

    A human-specific regulatory mechanism revealed in a pre-implantation model. Fueyo R, Wang S, Crocker OJ, Swigut T, Nakauchi H, Wysocka J. Nature. 2025 Oct 1. doi: 10.1038/s41586-025-09571-1. 10.1038/s41586-025-09571-1 PubMed 41034587
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