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pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro
(Plasmid #248161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248161 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    piggyBac
  • Total vector size (bp) 12373
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    KRAB-dCas9-HA-tagBFP
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    5234
  • Promoter Ef1a
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • tagBFP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site AsISI (not destroyed)
  • 5′ sequencing primer M13 fw GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    HA-CymR-T2A-puro
  • Species
    Synthetic
  • Insert Size (bp)
    1503
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro was a gift from Joanna Wysocka (Addgene plasmid # 248161 ; http://n2t.net/addgene:248161 ; RRID:Addgene_248161)

  • For your References section:

    A human-specific regulatory mechanism revealed in a pre-implantation model. Fueyo R, Wang S, Crocker OJ, Swigut T, Nakauchi H, Wysocka J. Nature. 2025 Oct 1. doi: 10.1038/s41586-025-09571-1. 10.1038/s41586-025-09571-1 PubMed 41034587
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