Skip to main content
Addgene
Open Menu

pB-Ef1a-HERVK-proteins
(Plasmid #248162)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248162 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    piggyBac
  • Total vector size (bp) 11732
  • Vector type
    Mammalian Expression
  • Selectable markers
    tagBFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HERVK GAG-PRO-POL
  • Species
    H. sapiens (human)
  • Promoter Ef1a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer M13 fw GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tagBFP
  • Species
    Synthetic
  • Promoter Ef1a

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer M13 fw GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-Ef1a-HERVK-proteins was a gift from Joanna Wysocka (Addgene plasmid # 248162 ; http://n2t.net/addgene:248162 ; RRID:Addgene_248162)

  • For your References section:

    A human-specific regulatory mechanism revealed in a pre-implantation model. Fueyo R, Wang S, Crocker OJ, Swigut T, Nakauchi H, Wysocka J. Nature. 2025 Oct 1. doi: 10.1038/s41586-025-09571-1. 10.1038/s41586-025-09571-1 PubMed 41034587
Related items: