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pET28_WT-LGP2 (codon optimized)
(Plasmid #248163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248163 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone size w/o insert (bp) 5598
  • Total vector size (bp) 7632
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For plasmid amplification, grow in DH5α; for protein expression, use Rosetta 2 (DE3) cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DExH-box helicase 58 (DHX58)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2034
  • Entrez Gene
    DHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
  • Tag / Fusion Protein
    • 6xHis, SUMO (N terminal on insert)

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer TTATATTTCCAAGGTATGGAACTGCGTAGCTATCAATGGG
  • 3′ sequencing primer TGCGGCCGCAAGCTTTTAGTCCAGGCTCAGGTCGCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_WT-LGP2 (codon optimized) was a gift from Smita Patel (Addgene plasmid # 248163 ; http://n2t.net/addgene:248163 ; RRID:Addgene_248163)

  • For your References section:

    Unraveling blunt-end RNA binding and ATPase-driven translocation activities of the RIG-I family helicase LGP2. Lee KY, Craig C, Patel SS. Nucleic Acids Res. 2024 Jan 11;52(1):355-369. doi: 10.1093/nar/gkad1106. 10.1093/nar/gkad1106 PubMed 38015453
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