pR6K-mScarI
(Plasmid
#248166)
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PurposeR6K plasmid with constituitive red fluorescent protein (mScarlet-I) expression under J23101 promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneR6K-KanR
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)SHARK2
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Growth instructionsAddgene strain #248173
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemScarlet-I
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SpeciesSynthetic
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MutationNone
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Please visit https://doi.org/10.1101/2025.10.06.680659 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pR6K-mScarI was a gift from Thomas Gorochowski (Addgene plasmid # 248166 ; http://n2t.net/addgene:248166 ; RRID:Addgene_248166) -
For your References section:
SHARK: A Specialized Host for Assembling R6K Plasmids. Hina-Nilesh Joshi S, Jenkins C, Ulaeto D, Gorochowski TE. ACS Synth Biol. 2026 Jan 13. doi: 10.1021/acssynbio.5c00749. 10.1021/acssynbio.5c00749 PubMed 41529903