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pRESTA-FLAG-CLIP-3XTPR-SNAP-StrepTag
(Plasmid #248184)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248184 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRESTA
  • Backbone size w/o insert (bp) 2857
  • Vector type
    Bacterial Expression ; 1578

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-CLIP-3XTPR-SNAP-StrepTag
  • Species
    Synthetic
  • Insert Size (bp)
    1578
  • Mutation
    WT
  • Promoter T7
  • Tags / Fusion Proteins
    • HisTag, FLAG, CLIP (N terminal on insert)
    • SNAP, StrepTag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GeneArt (Invitrogen by ThermoFisher Scientific)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.03.05.709886 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRESTA-FLAG-CLIP-3XTPR-SNAP-StrepTag was a gift from Alison Twelvetrees (Addgene plasmid # 248184 ; http://n2t.net/addgene:248184 ; RRID:Addgene_248184)

  • For your References section:

    A universal protein ladder for standardisation of diverse FRET assays. Smith ER, Gelder KL, Hunter-Craig L, Bose DA, Craggs TD, Twelvetrees AE. bioRxiv 2026.03.05.709886 10.64898/2026.03.05.709886
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