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pcDNA3-Arl13b-PR-EGFP-TurboID
(Plasmid #248196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248196 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 7308
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    The Arl13b noncilium targeting sequence-EGFP-TurboID
  • Alt name
    Arl13b nonCTS- EGFP-TurboID
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1926
  • Mutation
    Truncated to only contain the PR domain
  • Entrez Gene
    Arl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EGFP (C terminal on insert)
    • TurboID (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Arl13b-PR-EGFP-TurboID was a gift from Xuecai Ge (Addgene plasmid # 248196 ; http://n2t.net/addgene:248196 ; RRID:Addgene_248196)

  • For your References section:

    Numb positively regulates Hedgehog signaling at the ciliary pocket. Liu X, Yam PT, Schlienger S, Cai E, Zhang J, Chen WJ, Torres Gutierrez O, Jimenez Amilburu V, Ramamurthy V, Ting AY, Branon TC, Cayouette M, Gen R, Marks T, Kong JH, Charron F, Ge X. Nat Commun. 2024 Apr 25;15(1):3365. doi: 10.1038/s41467-024-47244-1. 10.1038/s41467-024-47244-1 PubMed 38664376
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