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EKv376(gRNAscaffold-tRNA for cloning)
(Plasmid #248333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248333 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNAscaffold-tRNA
  • Species
    P. patens

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golden Gate fragments are amplified by PCR, with primers designed to produce half of each gRNA followed by BsaI recognition sites. Complete gRNA sequences to be reconstituted upon BsaI digestion and ligation during the Golgen Gate reaction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EKv376(gRNAscaffold-tRNA for cloning) was a gift from Elena Kozgunova (Addgene plasmid # 248333 ; http://n2t.net/addgene:248333 ; RRID:Addgene_248333)

  • For your References section:

    tRNA-Based Polycistronic CRISPR/Cas9 System Boosts Efficiency of Multi-Gene Deletion in the Moss Physcomitrella. Kozgunova E. bioRxiv 2025.07.29.667574 10.1101/2025.07.29.667574
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