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EKv408(Gold Cas-guide insert4)
(Plasmid #248337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248337 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Intermediate vector, referred to as a gRNA insert vector, is used to clone guide RNA sequences by annealing complementary oligonucleotides with appropriate overhangs and inserting them into a BbsI site positioned between the PpU6 promoter and the gRNA scaffold. The PpU6 promoter and gRNA scaffold are flanked by outward-facing BsaI sites with unique four-base overhangs, enabling directional and ordered assembly via Golden Gate.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EKv408(Gold Cas-guide insert4) was a gift from Elena Kozgunova (Addgene plasmid # 248337 ; http://n2t.net/addgene:248337 ; RRID:Addgene_248337)

  • For your References section:

    tRNA-Based Polycistronic CRISPR/Cas9 System Boosts Efficiency of Multi-Gene Deletion in the Moss Physcomitrella. Kozgunova E. bioRxiv 2025.07.29.667574 10.1101/2025.07.29.667574
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