pEVBC1_moxBFP
(Plasmid
#248345)
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PurposeBlue fluorescent protein control for Fluorescent Protein Library
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEVBC1
- Total vector size (bp) 2808
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemoxBFP
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SpeciesSynthetic
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Insert Size (bp)726
- Promoter P_LacUV5
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer TGTGAGCGGATAACAATTTCACACAGGAAACAGCTCATATG
- 3′ sequencing primer CGAAAAGTGCCACCTGACGTCGTGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Backbone pEVBC1 is derived from pUC19.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEVBC1_moxBFP was a gift from Calin Plesa (Addgene plasmid # 248345 ; http://n2t.net/addgene:248345 ; RRID:Addgene_248345)