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pEVBC1_moxBFP
(Plasmid #248345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248345 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEVBC1
  • Total vector size (bp) 2808
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    moxBFP
  • Species
    Synthetic
  • Insert Size (bp)
    726
  • Promoter P_LacUV5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer TGTGAGCGGATAACAATTTCACACAGGAAACAGCTCATATG
  • 3′ sequencing primer CGAAAAGTGCCACCTGACGTCGTGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone pEVBC1 is derived from pUC19.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVBC1_moxBFP was a gift from Calin Plesa (Addgene plasmid # 248345 ; http://n2t.net/addgene:248345 ; RRID:Addgene_248345)

  • For your References section:

    Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning. Romanowicz KJ, Resnick C, Hinton SR, Plesa C. Sci Adv. 2025 Aug 15;11(33):eadw9178. doi: 10.1126/sciadv.adw9178. Epub 2025 Aug 13. 10.1126/sciadv.adw9178 PubMed 40802757
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