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pET21a-irisin
(Plasmid #248349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248349 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Low temperature expression of proteins in BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    irisin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    309
  • Entrez Gene
    FNDC5 (a.k.a. FRCP2, irisin)
  • Tag / Fusion Protein
    • His

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-irisin was a gift from Yanjun Dong (Addgene plasmid # 248349 ; http://n2t.net/addgene:248349 ; RRID:Addgene_248349)

  • For your References section:

    Irisin regulates the phosphorylation of glucocorticoid receptor Ser212 and Ser234 and mediates glucocorticoid-induced muscle atrophy in mice. Yang J, Zhang P, An Y, Tan S, Sun S, Liu Y, Zhu L, Wang H, Gao T, Dong Y. Commun Biol. 2025 Dec 4;8(1):1746. doi: 10.1038/s42003-025-09203-4. 10.1038/s42003-025-09203-4 PubMed 41345463
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